Freshney's Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications 8th Edition
$10.00R. Ian Freshney, Amanda Capes-Davis
ISBNs: 1119513014, 978-1119513018, 9781119513018, 9781119513032 , 9781119513049, 978-1119513032 , 978-1119513049, B08ZJD1ZRB
English | 2021 | Original PDF | 835 Pages
THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL
CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS,
CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES
Freshney’s Culture of
Animal Cells is the most comprehensive and up-to-date resource on the
principles, techniques, equipment, and applications in the field of
cell and tissue culture. Explaining both how to do tissue culture and
why a technique is done in a particular way, this classic text covers
the biology of cultured cells, how to select media and substrates,
regulatory requirements, laboratory protocols, aseptic technique,
experimental manipulation of animal cells, and much more.
The
eighth edition contains extensively revised material that reflects the
latest techniques and emerging applications in cell culture, such as
the use of CRISPR/Cas9 for gene editing and the adoption of chemically
defined conditions for stem cell culture. A brand-new chapter examines
the origin and evolution of cell lines, joined by a dedicated chapter
on irreproducible research, its causes, and the importance of
reproducibility and good cell culture practice. Throughout the book,
updated chapters and protocols cover topics including live-cell
imaging, 3D culture, scale-up and automation, microfluidics,
high-throughput screening, and toxicity testing. This landmark text:
Provides
comprehensive single-volume coverage of basic skills and protocols,
specialized techniques and applications, and new and emerging
developments in the field
Covers every essential area of animal cell
culture, including lab design, disaster and contingency planning,
safety, bioethics, media preparation, primary culture, mycoplasma and
authentication testing, cell line characterization and
cryopreservation, training, and troubleshooting
Features a wealth of
new content including protocols for gene delivery, iPSC generation and
culture, and tumor spheroid formation
Includes an updated and
expanded companion website containing figures, artwork, and
supplementary protocols to download and print
The eighth edition
of Freshney’s Culture of Animal Cells is an indispensable volume for
anyone involved in the field, including undergraduate and graduate
students, clinical and biopharmaceutical researchers, bioengineers,
academic research scientists, and managers, technicians, and trainees
working in cell biology, molecular biology, and genetics laboratories.
